A supervisor you don't usually work with asks you to do something that seems to conflict with your normal procedure. What should you do?

Procedural discrepancies between supervisors create genuine ambiguity. The professional response is to seek clarification calmly — not to ignore the instruction (insubordination) or follow it blindly without understanding (potential error). Stating what you normally do and asking whether the supervisor is intentionally changing the approach is both respectful and practical. If still uncertain, checking with your regular supervisor is appropriate.